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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
Annexin V Apc/7 Aad Apoptosis Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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Beyotime apoptosis analysis kit
10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
Apoptosis Analysis Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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10 wt% PCL/nHA enhances cell proliferation and reduces <t>apoptosis</t> via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.
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Image Search Results


10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

Journal: Bioactive Materials

Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

doi: 10.1016/j.bioactmat.2026.03.037

Figure Lengend Snippet: 10 wt% PCL/nHA enhances cell proliferation and reduces apoptosis via calcium ion regulation. (A) Morphological characteristics and viability of L929 cells cultured on PCL and PCL/nHA samples. (B) Statistical evaluation of L929 cells viability. (C) Morphological characteristics and viability of HSFs cultured on PCL and PCL/nHA samples. (D) Statistical evaluation of HSFs viability. (E) Time-dependent proliferation of L929 cells on PCL and PCL/nHA substrates over 1, 2, and 3 days. (F) Time-dependent proliferation of HSFs on PCL and PCL/nHA substrates over 1, 2, and 3 days. (G) Influence of varying nHA concentrations in PCL on L929 cells apoptosis. (H) Quantitative apoptosis assessment across experimental groups based on data from G. (I) Apoptosis of L929 cells cultured in media with varying calcium ion concentrations. (J) Statistical analysis of the apoptosis data presented in I. (K) The morphological characteristics and viability of L929 cells cultured in the CaCl 2 group, the nHA group (with calcium ion concentration equivalent to that in the CaCl 2 group), and the control group. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

Article Snippet: CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China).

Techniques: Cell Culture, Concentration Assay, Control

Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

Journal: Bioactive Materials

Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion

doi: 10.1016/j.bioactmat.2026.03.037

Figure Lengend Snippet: Piezo1-mediated Ca 2+ signaling in PCL/nHA-Driven cell behaviors. (A) Fluorescence imaging of intracellular calcium ion influx in L929 cells on PCL and PCL/nHA surfaces. (B) Fluorescence quantification of calcium ion influx in A. (C) Fluorescence imaging of intracellular calcium ion influx in HSFs on PCL and PCL/nHA surfaces. (D) Fluorescence quantification of calcium ion influx in C. (E) Fluorescence imaging assessment of intracellular ROS levels in L929 cells on PCL and PCL/nHA samples. (F) Effects of PCL samples with different nHA contents on the mitochondrial membrane potential of L929 cells. (G) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (H) Assessment of the expression levels of mechanosensing- and proliferation-related proteins in HSFs cultured on PCL and PCL/nHA surfaces. (I) Assessment of the expression levels of apoptosis-related proteins in L929 cells cultured on PCL and PCL/nHA surfaces. (J) Schematic illustration depicting the mechanisms underlying L929 cell proliferation differences. All data are presented as the Mean ± SD (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, no significant difference.

Article Snippet: CCK-8 assay kit, Fluo-4AM, Cell Apoptosis Detection Kit, Mitochondrial membrane potential assay kit and ROS Assay Kit were purchased from Beyotime Biotechnology (China).

Techniques: Fluorescence, Imaging, Membrane, Expressing, Cell Culture